The Wnt pathway is a key intercellular signaling cascade that regulates development, tissue homeostasis, and regeneration. However, gaps remain in our understanding of the molecular events that take place between ligand-receptor binding and target gene transcription. We used a novel tool for quantitative, real-time assessment of endogenous pathway activation, measured in single cells, to answer an unresolved question in the field – whether receptor endocytosis is required for Wnt signal transduction. We combined knockdown or knockout of essential components of Clathrin-mediated endocytosis with quantitative assessment of Wnt signal transduction in mouse embryonic stem cells (mESCs).

Rat AGP ELISA Kit
E-25AGP 1 x 96 well plate
EUR 441

Chicken AGP ELISA Kit
E-30AGP 1 x 96 well plate
EUR 441

Dog AGP ELISA Kit
E-40AGP 1 x 96 well plate
EUR 492

Human AGP ELISA Kit
E-80AGP 1 x 96 well plate
EUR 370

IL-8 (-5 to +5)
5-01377 4 x 5mg Ask for price

Sheep A1-AGP ELISA Kit
ESA0695 96Tests
EUR 521

Mouse A1-AGP ELISA Kit
EMA0695 96Tests
EUR 521

Monkey A1-AGP ELISA Kit
EMKA0695 96Tests
EUR 521

Porcine A1-AGP ELISA Kit
EPA0695 96Tests
EUR 521

Rat A1-AGP ELISA Kit
ERA0695 96Tests
EUR 521

Rabbit A1-AGP ELISA Kit
ERTA0695 96Tests
EUR 521

Anserini A1-AGP ELISA Kit
EAA0695 96Tests
EUR 521

Goat A1-AGP ELISA Kit
EGTA0695 96Tests
EUR 521

Canine A1-AGP ELISA Kit
ECA0695 96Tests
EUR 521

Chicken A1-AGP ELISA Kit
ECKA0695 96Tests
EUR 521

Human A1-AGP ELISA Kit
EHA0695 96Tests
EUR 521

Bovine A1-AGP ELISA Kit
EBA0695 96Tests
EUR 521

AGP ELISA Kit (Bovine) (OKCD06846)
OKCD06846 96 Wells
EUR 1040
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL

2',3',5'-triacetyl-5-Azacytidine
C4119-5 5 mg
EUR 118
Description: 2',3',5'-triacetyl-5-Azacytidine (TAC) is the lead prodrug form of 5-azacytidine which may be rapidly absorbed after oral administration [1]. 5-Azacytidine is an inhibitor of DNA methyltransferase [2].

2',3',5'-triacetyl-5-Azacytidine
B2822-5
EUR 153

Histatin 5
5-01307 4 x 1mg Ask for price

5-Iodotubercidin
A3125-5 5 mg
EUR 168
Description: 5-Iodotubercidin (Itu) is a purine derivative and hence an inhibitor of adenosine kinase with an IC50 value of 26 nM [1]. Adenosine kinase is important in regulating the intracellular and extracellular concentrations of adenosine and hence diverse physiological actions of adenosine [2].

5-ROX
B8212-5 5 mg
EUR 195

GNF-5
B1329-5
EUR 142

Necrostatin-5
1865-5
EUR 126

Aftin-5
2575-5
EUR 457

CHD-5
2693-5
EUR 153

Guinea Pig A1-AGP ELISA Kit
EGA0695 96Tests
EUR 521

Thrombin Receptor Activator for Peptide 5 (TRAP-5)
A1036-5 5 mg
EUR 102
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.

TRAP-5 amide
5-02019 4 x 5mg Ask for price

[Cit5]-TRAP-5
5-00062 4 x 5mg Ask for price

A-VI-5
5-00524 4 x 5mg Ask for price

ACTH(5-10)
5-00605 4 x 5mg Ask for price

Angiotensin (1-5)
5-00676 4 x 5mg Ask for price

Bradykinin (1-5)
5-00852 4 x 5mg Ask for price

W-5 hydrochloride
B6277-5 5 mg
EUR 116

5-Nonyloxytryptamine oxalate
B6496-5 5 mg
EUR 154

5'-deoxy Thymidine
C4692-5 5 mg
EUR 180
Description: 5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines.

Teicoplanin A2-5
C4938-5 5 mg
EUR 1157
Description: Teicoplanin A2-5, derived from Actinoplanes teichomyceticus, is a glycopeptide antibiotic which produces a broad spectrum of antibiotic activity against gram-positive bacteria.

5-fluoro 203
C4114-5 5 mg
EUR 154
Description: 5-fluoro 203 (5F-203) is an antitumor agent and cytotoxic compound that acts as a potent AhR agonist [1][2][3]. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in regulating xenobiotic-metabolizing enzymes such as cytochrome P450.

2',5'-Dideoxyadenosine
2770-5
EUR 240

5-Formylcytosine (5-fC) ELISA Kit
MET-5102-5 5 x 96 assays
EUR 2283

5-Carboxylcytosine (5-caC) ELISA Kit
MET-5103-5 5 x 96 assays
EUR 2283

Secretin (5 - 27), porcine
5-01917 4 x 1mg Ask for price

SH2 Domain Ligand (5)
5-01929 4 x 5mg Ask for price

Boc-5-Ava-OH
5-04607 25g Ask for price

Boc-5-Ava-OH
5-04608 100g Ask for price

Fmoc-5-Ava-OH
5-04609 5g Ask for price

Fmoc-5-Ava-OH
5-04610 25g Ask for price

Dok- 5 (263 - 275)
5-01048 4 x 5mg Ask for price

5'-(N-Cyclopropyl)carboxamidoadenosine
C4645-5 5 mg
EUR 128

2',5'-dideoxy Adenosine
C3993-5 5 mg
EUR 189
Description: 2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1]. Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells.

FAM azide, 5- isomer
A8117-5 5 mg
EUR 166
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

ROX azide, 5- isomer
A8126-5 5 mg
EUR 583
Description: ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.Pure 5-isomer.

TAMRA azide, 5- isomer
A8129-5 5 mg
EUR 583
Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.TAMRA is often used as FRET acceptor for FAM fluorophore.

5-trans U-46619
C4261-5 5 mg
EUR 538
Description: 5-trans U-46619 is a minor impurity (2-5%) which variably exists in most commercial preparations of U-46619 [1]. U-46619 is a selective thromboxane A2 agonist involved in inducing platelet aggregation and vascular smooth muscle contraction [2].

Individual Reaction Mix 5
G065-5 200 reactions
EUR 167

5-Nitroso-8-quinolinol
B2825-5
EUR 109

AICA riboside, 5'-phosphate
9416-5
EUR 153

Necrosis Inhibitor, Necrox-5
2229-5
EUR 615

Alpha 1 Acid Glycoprotein (A1-AGP), bovine
A1AG20-N 1 mg
EUR 164

Alpha 1 Acid Glycoprotein (A1-AGP), Rabbit
A1AG23-N-25 25 ug
EUR 286

Alpha 1 Acid Glycoprotein (A1-AGP), Turkey
A1AG24-N-100 100 ug
EUR 164

anti-α1-acid glycoprotein (AGP)(29A1)
LF-MA0167 100 ul
EUR 334
Description: Mouse monoclonal to α1-acid glycoprotein (AGP)

Fmoc-Trp(5-OH)-OH
5-06939 5g Ask for price

Fmoc-Trp(5-OH)-OH
5-06940 25g Ask for price

[Pyr5]-Substance P (5-11)
5-00288 4 x 5mg Ask for price

[Trp3, Arg5]-Ghrelin (1-5)
5-00313 4 x 5mg Ask for price

beta-Casomorphin (1-5) (bovine)
5-00475 4 x 5mg Ask for price

Angiotensin I/II (5-8)
5-00684 4 x 5mg Ask for price

Cholecystokinin Octapeptide (1-5) (desulfated)
5-00970 4 x 5mg Ask for price

HIV-gp41-Antigenic Peptide 5
5-01331 4 x 1mg Ask for price

Magnetic Beads (DNA) 5 mL
P920-5 - Ask for price

Angiotensin 1/2 (1-5)
A1047-5 5 mg
EUR 119
Description: Angiotensin I/II (1-5) is a peptide (ASP-ARG-VAL-TYR-ILE) that contains the amino acids 1-5 and is converted from Angiotensin I/II.Angiotensin I is formed by the action ofreninonangiotensinogen, an ?-2-globulin with 12 amino acids.

Angiotensin 1/2 (5-7)
A1049-5 5 mg
EUR 119
Description: Angiotensin 1/2 (5-7), (C17H27N5O4), a peptide with the sequence H2N-Ile-His-Pro-OH, MW=365.43. Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure.

Helioxanthin derivative 5-4-2
B4700-5 5 mg
EUR 683
Description: Helioxanthin derivative 5-4-2, an analogue of helioxanthin (ACH126447), is a small-molecule inhibitor of HBV, HSV-1and HSV-2 virus with EC50 values of 0.08 ?M, 0.29 ?M and 0.16 ?M, respectively [1].

2'-O-Methylpseudouridine-5'-Triphosphate
B7993-.5 5x100 µl (100 mM)
EUR 14023

2'-Fluoro-thymidine-5'-Triphosphate
B8035-.5 5x100 µl (100 mM)
EUR 2059

3,3',5'-Triiodo-L-thyronine
C5014-5 5 mg
EUR 134
Description: 3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TR? and TR? antagonist [1].

5?-dihydro-11-keto Testosterone
C4317-5 5 mg
EUR 586
Description: 5?-dihydro-11-keto Testosterone (11KDHT) is a full androgen receptor agonist comparable to dihydrotestosterone (DHT) [1][2][3].

3,3',5'-Triiodo-L-thyronine
B2017-5
EUR 229

2-Methyl-5-hydroxytryptamine hydrochloride
2768-5
EUR 185

pLenti-CLDN1 shRNA-5 Plasmid
PVTBAV04867-5 2 ug
EUR 356

Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
GA-E0068PC-48T 48T
EUR 364

Porcine α1-Acid glycoprotein,α1-AGP ELISA Kit
GA-E0068PC-96T 96T
EUR 590

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E0093RT-48T 48T
EUR 317

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E0093RT-96T 96T
EUR 496

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E1792HM-48T 48T
EUR 289

Human α1-Acid glycoprotein(α1-AGP)ELISA Kit
GA-E1792HM-96T 96T
EUR 466

Human ?1-Acid glycoprotein,?1-AGP ELISA Kit
201-12-1776 96 tests
EUR 440
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human Alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody
12011-05011 150 ug
EUR 217

Human alpha-1-Acid-Glycoprotein (Orosomucoid, AGP) Antibody
32220-05111 150 ug
EUR 261

Alpha 1 Acid Glycoprotein (A1-AGP), Human Plasma
A1AG15-N 1 mg
EUR 164

Mouse α1-AGP(α1-Acid glycoprotein) ELISA Kit
EM1448 96T
EUR 524.1
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 3.75 ng/ml

Porcine α1-AGP(α1-Acid Glycoprotein) ELISA Kit
EP0214 96T
EUR 567.6
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Pig;Sensitivity: 0.094 ng/ml

Rat α1-Acid glycoprotein(α1-AGP)ELISA Kit
QY-E11560 96T
EUR 361

Disruption of Clathrin-mediated endocytosis did not affect accumulation and nuclear translocation of β-catenin, as measured by single-cell live imaging of endogenous β-catenin, and subsequent target gene transcription. Disruption of another receptor endocytosis pathway, Caveolin-mediated endocytosis, did not affect Wnt pathway activation in mESCs. Additional results in multiple cell lines support that endocytosis is not a requirement for Wnt signal transduction. We show that off-target effects of a drug used to inhibit endocytosis may be one source of the discrepancy among reports on the role of endocytosis in Wnt signaling.