The Wnt pathway is a key intercellular signaling cascade that regulates development, tissue homeostasis, and regeneration. However, gaps remain in our understanding of the molecular events that take place between ligand-receptor binding and target gene transcription. We used a novel tool for quantitative, real-time assessment of endogenous pathway activation, measured in single cells, to answer an unresolved question in the field – whether receptor endocytosis is required for Wnt signal transduction. We combined knockdown or knockout of essential components of Clathrin-mediated endocytosis with quantitative assessment of Wnt signal transduction in mouse embryonic stem cells (mESCs).

Rat AGP ELISA Kit

E-25AGP 1 x 96 well plate
EUR 529.2

Chicken AGP ELISA Kit

E-30AGP 1 x 96 well plate
EUR 529.2

Dog AGP ELISA Kit

E-40AGP 1 x 96 well plate
EUR 590.4

Human AGP ELISA Kit

E-80AGP 1 x 96 well plate
EUR 444

IL-8 (-5 to +5)

5-01377 4 x 5mg Ask for price

2',3',5'-triacetyl-5-Azacytidine

B2822-5
EUR 183.6

2',3',5'-triacetyl-5-Azacytidine

C4119-5 5 mg
EUR 141.6
Description: 2',3',5'-triacetyl-5-Azacytidine (TAC) is the lead prodrug form of 5-azacytidine which may be rapidly absorbed after oral administration [1]. 5-Azacytidine is an inhibitor of DNA methyltransferase [2].

Rabbit A1-AGP ELISA Kit

ERTA0695 96Tests
EUR 625.2

Rat A1-AGP ELISA Kit

ERA0695 96Tests
EUR 625.2

Mouse A1-AGP ELISA Kit

EMA0695 96Tests
EUR 625.2

Monkey A1-AGP ELISA Kit

EMKA0695 96Tests
EUR 625.2

Porcine A1-AGP ELISA Kit

EPA0695 96Tests
EUR 625.2

AGP ELISA Kit (Bovine) (OKCD06846)

OKCD06846 96 Wells
EUR 1248
Description: Description of target: Functions as transport protein in the blood stream.UniRule annotation;Species reactivity: Bovine;Application: ELISA;Assay info: ;Sensitivity: < 7.0ng/mL

Human A1-AGP ELISA Kit

EHA0695 96Tests
EUR 625.2

Goat A1-AGP ELISA Kit

EGTA0695 96Tests
EUR 625.2

Canine A1-AGP ELISA Kit

ECA0695 96Tests
EUR 625.2

Chicken A1-AGP ELISA Kit

ECKA0695 96Tests
EUR 625.2

Anserini A1-AGP ELISA Kit

EAA0695 96Tests
EUR 625.2

Bovine A1-AGP ELISA Kit

EBA0695 96Tests
EUR 625.2

Sheep A1-AGP ELISA Kit

ESA0695 96Tests
EUR 625.2

Histatin 5

5-01307 4 x 1mg Ask for price

Necrostatin-5

1865-5
EUR 151.2

Aftin-5

2575-5
EUR 548.4

CHD-5

2693-5
EUR 183.6

5-Iodotubercidin

A3125-5 5 mg
EUR 201.6
Description: 5-Iodotubercidin (Itu) is a purine derivative and hence an inhibitor of adenosine kinase with an IC50 value of 26 nM [1]. Adenosine kinase is important in regulating the intracellular and extracellular concentrations of adenosine and hence diverse physiological actions of adenosine [2].

GNF-5

B1329-5
EUR 170.4

5-ROX

B8212-5 5 mg
EUR 234

Thrombin Receptor Activator for Peptide 5 (TRAP-5)

A1036-5 5 mg
EUR 122.4
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.

[Cit5]-TRAP-5

5-00062 4 x 5mg Ask for price

A-VI-5

5-00524 4 x 5mg Ask for price

ACTH(5-10)

5-00605 4 x 5mg Ask for price

Angiotensin (1-5)

5-00676 4 x 5mg Ask for price

Bradykinin (1-5)

5-00852 4 x 5mg Ask for price

TRAP-5 amide

5-02019 4 x 5mg Ask for price

2',5'-Dideoxyadenosine

2770-5
EUR 288

W-5 hydrochloride

B6277-5 5 mg
EUR 139.2

5-Nonyloxytryptamine oxalate

B6496-5 5 mg
EUR 184.8

5'-deoxy Thymidine

C4692-5 5 mg
EUR 216
Description: 5'-Deoxy Thymidine has been used as a research tool for antiviral and anticancer studies. 5'-Deoxy Thymidine can be used to study the entry mechanism into human erythrocytes in comparison with other deoxythymidines.

Teicoplanin A2-5

C4938-5 5 mg
EUR 1388.4
Description: Teicoplanin A2-5, derived from Actinoplanes teichomyceticus, is a glycopeptide antibiotic which produces a broad spectrum of antibiotic activity against gram-positive bacteria.

5-fluoro 203

C4114-5 5 mg
EUR 184.8
Description: 5-fluoro 203 (5F-203) is an antitumor agent and cytotoxic compound that acts as a potent AhR agonist [1][2][3]. The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor involved in regulating xenobiotic-metabolizing enzymes such as cytochrome P450.

Guinea Pig A1-AGP ELISA Kit

EGA0695 96Tests
EUR 625.2

Nori® Monkey AGP ELISA Kit

GR169206 96-well
EUR 553.2

Nori® Hamster AGP ELISA Kit

GR172256 96-well
EUR 553.2

Nori® Zebrafish AGP ELISA Kit

GR206130 96-well
EUR 553.2

Nori® Ferret AGP ELISA Kit

GR201130 96-well
EUR 553.2

Nori® Salmon AGP ELISA Kit

GR202130 96-well
EUR 553.2

5-Formylcytosine (5-fC) ELISA Kit

MET-5102-5 5 x 96 assays
EUR 2739.6

5-Carboxylcytosine (5-caC) ELISA Kit

MET-5103-5 5 x 96 assays
EUR 2739.6

Boc-5-Ava-OH

5-04607 25g Ask for price

Boc-5-Ava-OH

5-04608 100g Ask for price

Fmoc-5-Ava-OH

5-04609 5g Ask for price

Fmoc-5-Ava-OH

5-04610 25g Ask for price

Dok- 5 (263 - 275)

5-01048 4 x 5mg Ask for price

Secretin (5 - 27), porcine

5-01917 4 x 1mg Ask for price

SH2 Domain Ligand (5)

5-01929 4 x 5mg Ask for price

Necrosis Inhibitor, Necrox-5

2229-5
EUR 738

FAM azide, 5- isomer

A8117-5 5 mg
EUR 199.2
Description: FAM azide for Click chemistry labeling. FAM remains one of the most popular fluorescent lablels for various application. Most instruments capable of fluorescence detection, ranging from plate readers to fluorescence microscopes, are able to work in FAM channel.

ROX azide, 5- isomer

A8126-5 5 mg
EUR 699.6
Description: ROX (rhodamine-X) azide, solid material, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. ROX is a red-emitting rhodamine dye possessing high brightness and fluorescence quantum yield.Pure 5-isomer.

TAMRA azide, 5- isomer

A8129-5 5 mg
EUR 699.6
Description: TAMRA (TMR, tetramethylrhodamine) azide, solid compound, and 10 mM solution in DMSO, labeling reagent for Click Chemistry. Pure 5-isomer.TAMRA is often used as FRET acceptor for FAM fluorophore.

AICA riboside, 5'-phosphate

9416-5
EUR 183.6

5-Nitroso-8-quinolinol

B2825-5
EUR 130.8

5'-(N-Cyclopropyl)carboxamidoadenosine

C4645-5 5 mg
EUR 153.6

5-trans U-46619

C4261-5 5 mg
EUR 645.6
Description: 5-trans U-46619 is a minor impurity (2-5%) which variably exists in most commercial preparations of U-46619 [1]. U-46619 is a selective thromboxane A2 agonist involved in inducing platelet aggregation and vascular smooth muscle contraction [2].

2',5'-dideoxy Adenosine

C3993-5 5 mg
EUR 226.8
Description: 2',5'-dideoxy Adenosine, a nucleoside analog, is one of the first identified cell-permeable, P-site inhibitors of adenylate cyclase [1]. Adenylyl cyclase is an enzyme with key regulatory roles in almost all cells.

Individual Reaction Mix 5

G065-5 200 reactions
EUR 200.4

Alpha 1 Acid Glycoprotein (A1-AGP), bovine

A1AG20-N 1 mg
EUR 196.8

Alpha 1 Acid Glycoprotein (A1-AGP), Rabbit

A1AG23-N-25 25 ug
EUR 343.2

Alpha 1 Acid Glycoprotein (A1-AGP), Turkey

A1AG24-N-100 100 ug
EUR 196.8

anti-α1-acid glycoprotein (AGP)(29A1)

LF-MA0167 100 ul
EUR 400.8
Description: Mouse monoclonal to α1-acid glycoprotein (AGP)

Fmoc-Trp(5-OH)-OH

5-06939 5g Ask for price

Fmoc-Trp(5-OH)-OH

5-06940 25g Ask for price

Angiotensin 1/2 (1-5)

A1047-5 5 mg
EUR 142.8
Description: Angiotensin I/II (1-5) is a peptide (ASP-ARG-VAL-TYR-ILE) that contains the amino acids 1-5 and is converted from Angiotensin I/II.Angiotensin I is formed by the action ofreninonangiotensinogen, an ?-2-globulin with 12 amino acids.

Angiotensin 1/2 (5-7)

A1049-5 5 mg
EUR 142.8
Description: Angiotensin 1/2 (5-7), (C17H27N5O4), a peptide with the sequence H2N-Ile-His-Pro-OH, MW=365.43. Angiotensin is a peptide hormone that causes vasoconstriction and a subsequent increase in blood pressure.

[Pyr5]-Substance P (5-11)

5-00288 4 x 5mg Ask for price

[Trp3, Arg5]-Ghrelin (1-5)

5-00313 4 x 5mg Ask for price

beta-Casomorphin (1-5) (bovine)

5-00475 4 x 5mg Ask for price

Angiotensin I/II (5-8)

5-00684 4 x 5mg Ask for price

Cholecystokinin Octapeptide (1-5) (desulfated)

5-00970 4 x 5mg Ask for price

HIV-gp41-Antigenic Peptide 5

5-01331 4 x 1mg Ask for price

2-Methyl-5-hydroxytryptamine hydrochloride

2768-5
EUR 222

2'-O-Methylpseudouridine-5'-Triphosphate

B7993-.5 5x100 µl (100 mM)
EUR 16827.6

2'-Fluoro-thymidine-5'-Triphosphate

B8035-.5 5x100 µl (100 mM)
EUR 2470.8

Helioxanthin derivative 5-4-2

B4700-5 5 mg
EUR 819.6
Description: Helioxanthin derivative 5-4-2, an analogue of helioxanthin (ACH126447), is a small-molecule inhibitor of HBV, HSV-1and HSV-2 virus with EC50 values of 0.08 ?M, 0.29 ?M and 0.16 ?M, respectively [1].

3,3',5'-Triiodo-L-thyronine

B2017-5
EUR 274.8

3,3',5'-Triiodo-L-thyronine

C5014-5 5 mg
EUR 160.8
Description: 3,3',5'-triiodo-L-thyronine (reverse T3 or rT3) and 3,5,3'-triiodo-L-thyronine (T3) are the metabolism of thyroxine (T4) [1]. 3,3',5'-Triiodo-L-thyronine is thyroid hormone receptors TR? and TR? antagonist [1].

5?-dihydro-11-keto Testosterone

C4317-5 5 mg
EUR 703.2
Description: 5?-dihydro-11-keto Testosterone (11KDHT) is a full androgen receptor agonist comparable to dihydrotestosterone (DHT) [1][2][3].

Magnetic Beads (DNA) 5 mL

P920-5 - Ask for price

pLenti-CLDN1 shRNA-5 Plasmid

PVTBAV04867-5 2 ug
EUR 427.2

7078D 5 PIPET 5ML STERILE STERILE

7078D-5 25/pk
EUR 260.4
Description: Disposable Pipets; Disposable Pipets, Serological, Misc

Fmoc-D-Trp(5-Br)-OH

5-04217 1g Ask for price

Fmoc-D-Trp(5-Br)-OH

5-04218 5g Ask for price

Fmoc-D-Trp(5-Cl)-OH

5-04223 1g Ask for price

Fmoc-D-Trp(5-Cl)-OH

5-04224 5g Ask for price

Fmoc-D-Trp(5-F)-OH

5-04227 1g Ask for price

Fmoc-D-Trp(5-F)-OH

5-04228 5g Ask for price

Ac-DL-Trp(5-Br)-OH·DCHA

5-04229 1g Ask for price

Ac-DL-Trp(5-Br)-OH·DCHA

5-04230 5g Ask for price

Ac-DL-Trp(5-Cl)-OH·DCHA

5-04231 1g Ask for price

Ac-DL-Trp(5-Cl)-OH·DCHA

5-04232 5g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid

5-04549 1g Ask for price

Fmoc-2-amino-5-Methoxybenzoic acid

5-04550 5g Ask for price

Fmoc-3-Ala(5-Thiazoyl)-OH

5-05361 1g Ask for price

Fmoc-3-Ala(5-Thiazoyl)-OH

5-05362 5g Ask for price

Beta-Sheet Breaker Peptide iA?5

A1005-5 5 mg
EUR 122.4
Description: Beta-Sheet Breaker Peptide iA?5 (C33H43N5O8), with the sequence H-Leu-Pro-Phe-Phe-Asp-OH, has been shown to inhibit amyloidogenesis in rat brain models.

beta-Casomorphin (1-5) amide (bovine)

5-00476 4 x 5mg Ask for price

cAMP Dependent PK Inhibitor (5-24)

5-00922 4 x 1mg Ask for price

Kinase Domain of Insulin Receptor (5)

5-01429 4 x 1mg Ask for price

2'-Fluoro-2'-deoxyadenosine-5'-Triphosphate

B7959-.5 5x100 µl (100 mM)
EUR 2470.8

2'-Fluoro-2'-deoxycytidine-5'-Triphosphate

B7961-.5 5x100 µl (100 mM)
EUR 2470.8

2'-Fluoro-2'-deoxyguanosine-5'-Triphosphate

B7962-.5 5x100 µl (100 mM)
EUR 2470.8

Disruption of Clathrin-mediated endocytosis did not affect accumulation and nuclear translocation of β-catenin, as measured by single-cell live imaging of endogenous β-catenin, and subsequent target gene transcription. Disruption of another receptor endocytosis pathway, Caveolin-mediated endocytosis, did not affect Wnt pathway activation in mESCs. Additional results in multiple cell lines support that endocytosis is not a requirement for Wnt signal transduction. We show that off-target effects of a drug used to inhibit endocytosis may be one source of the discrepancy among reports on the role of endocytosis in Wnt signaling.